Coding
Part:BBa_K4275029:Design
Designed by: Haoming Qin Group: iGEM22_GreatBay_SCIE (2022-10-09)
eforRED-dockerin II
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 702
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The DocII domain is situated on the C terminal of the fusion construct whilst the eforRED chromoprotein is situated at the N terminal.
2. The fusion protein is designed to prove the high-affinity of DocII:CohII interaction.
Source
Echinopora forskaliana (eforRED chromoprotein)
Clostridium thermocellum (Dockerin-II domain)
References
1. Brás, Joana L.A., et al. “Escherichia Coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes.” Cellulases, 2012, pp. 395–415, 10.1016/b978-0-12-415931-0.00021-5.
2. Part: "Bba K592012 - Parts.Igem.Org". Parts.Igem.Org, 2022, https://parts.igem.org/Part:BBa_K592012.